How to Inject NAD+: Step-by-Step Instructions (Home Injection Safety Guide)

NAD+ is one of the most powerful tools for boosting energy, improving focus, and supporting healthy aging—but injections can feel intimidating if you’ve never done them before.

Don’t worry—this guide makes it simple.

We’ll walk you through everything step by step: how to mix your NAD+ vial, where and how to inject, how much to take, and how to do it safely at home. With just a little practice, you’ll have a routine that’s fast, low-pain, and highly effective—no clinic visit required.

What Is NAD+ And Why The Injectable Form?

NAD+ (nicotinamide adenine dinucleotide) is a vital coenzyme found in every cell of your body. It helps power your metabolism, supports DNA repair, and plays a key role in healthy aging and energy production.

While oral NAD supplements and nasal sprays are also available, many people choose injections for better bioavailability and faster results. When injected, NAD+ bypasses the digestive system, allowing more of the nutrient to reach your cells directly—which means quicker effects and greater impact.

How to Inject NAD+: Step-by-Step Guide

Injecting NAD+ may sound intimidating at first, but it’s a straightforward process—especially when using the subcutaneous method. With clean technique and a little practice, most people find it low-pain, quick, and easy to manage at home.

Follow the steps below to feel confident and stay safe.

Where to Inject: Best Sites

Subcutaneous injections go just under the skin (not into the muscle), usually in areas with some fat.

• Lower abdomen (at least 2 inches away from the belly button)
• Outer thigh
• Back of the upper arm
• Love handle area

IVY RX Pro Tip: Rotate injection sites regularly to avoid irritation or tissue buildup.

Injection Steps

• Wash your hands and clean the injection site with an alcohol swab.
• Draw the correct dose into the syringe from your mixed NAD+ vial.
• Pinch the skin at your chosen injection site.
• Insert the needle at a 45-degree angle for subcutaneous injections.
• Inject slowly and steadily, then remove the needle.
• Dispose of the syringe in a sharps container and clean the area again if needed.

How To Mix (Reconstitute) NAD+ Correctly#

Some NAD+ injections come in powder form and need to be mixed with bacteriostatic water before use. This is called reconstitution, and it’s a standard step when preparing many injectable peptides or coenzymes.

If your vial is powdered, here’s how to safely reconstitute it:

1. Wash your hands and clean your work surface.
2. Remove the caps from the NAD+ vial and bacteriostatic water.
3. Wipe the rubber stoppers with an alcohol swab.
4. Draw up the recommended amount of water (e.g. 5mL for a 500mg vial).
5. Inject the water slowly down the inside wall of the vial.
6. Gently swirl (don’t shake) until fully dissolved.
7. Label the vial with the date and store it in the refrigerator.

NAD+ Injection Dosage

There’s no universal dose for NAD+—your ideal amount depends on your goals, tolerance, and how your body responds over time. That said, most people start low and increase gradually, especially when using NAD+ for energy, mental clarity, or anti-aging support.

Typical starting range:

• 25–100mg per injection
• 1 to 3 times per week

If you’re just starting out, begin with 25–50mg once or twice per week and see how your body responds. From there, you can slowly increase the dose or frequency over the next few weeks.

Some users increase their dose to:

• 100mg per injection for more noticeable energy or recovery support
• 2–3x per week for consistent benefits, especially during periods of higher stress or training

What to monitor:

• Energy levels
• Mental clarity and focus
• Sleep quality
• Recovery time from workouts or stress

IVY RX Top Tip: Some people benefit from cycling NAD+ (e.g., 6–8 weeks on, then a short break), while others use it continuously at a lower maintenance dose. Your IVY RX specialist can help customize your dosing plan based on your lab work and goals.

Always talk to a medical professional before increasing your dose significantly—especially if you have pre-existing health conditions.

Should You Inject NAD+ At Home Or In-Clinic?

Both options—home injections and in-clinic treatments—have their pros and cons. The right choice depends on your comfort level, health needs, and lifestyle.

Home Injections:

• Convenient and time-saving
• More affordable over time
• Great for people who are comfortable self-injecting

In-Clinic Administration:

• Professional oversight and support
• Ideal for beginners or those with medical concerns
• Useful for high-dose or IV NAD+ protocols

Speak with your healthcare provider or wellness team before deciding. Many start in-clinic and transition to home use once confident.

Storage And Safety Tips For NAD+

Proper storage keeps your NAD+ potent and safe to use. Follow these key tips to avoid contamination or reduced effectiveness:

• Refrigerate after mixing: Store the reconstituted solution in the fridge (36–46°F / 2–8°C).
• Label your vial with the date you mixed it—NAD+ is typically stable for up to 30 days when refrigerated.
• Use sterile needles and syringes every time. Never reuse supplies.
• Avoid freezing and keep the vial out of direct sunlight or heat.
• Dispose of used needles in a sharps container—not in your household trash.

Common Side Effects and When to Seek Help

NAD+ injections are generally well-tolerated—but like any supplement or therapy, side effects can happen, especially as your body adjusts. Most are mild and temporary.

Common short-term side effects:

• Headache or light pressure in the head
• Mild nausea
• Flushing or a warm sensation
• Slight burning or stinging at the injection site
• Redness or swelling under the skin

These symptoms are often related to injection speed or hydration levels. Injecting too quickly can overwhelm the body’s processing of NAD+, especially in early stages. Hydrating before and after can help minimize symptoms.

Tips to reduce side effects:

• Inject slowly—over 30–60 seconds
• Stay well-hydrated
• Take a walk or light movement afterward to help circulation
• Try splitting your dose if discomfort persists

When to call your provider:

• Severe or persistent nausea
• Rash, itching, or swelling (possible allergic reaction)
• Chest tightness or trouble breathing
• Vision changes or dizziness

Remember: If something feels “off” or lingers beyond the first few doses, don’t push through—reach out. Your IVY RX specialist is there to guide you and help adjust your plan if needed.

Why IVY RX NAD+ Injections Are The Right Choice

Feeling your best shouldn’t require clinic visits, confusing instructions, or trial and error. At IVY RX, we make NAD+ therapy simple, supportive, and effective—right from home.

• Clinician-backed, high-quality NAD+ you can trust
• Easy-to-follow guidance for safe, confident self-injection
• Ongoing support to help you adjust and stay on track
  
  

Whether you’re boosting energy, supporting healthy aging, or just wanting to feel more like yourself again, IVY RX helps you take the next step—on your terms.

Explore our NAD+ injections today and start your wellness journey with confidence.

FAQs

Is NAD+ injection painful?

Most people find NAD+ injections surprisingly tolerable. Subcutaneous injections use a tiny needle and only go just under the skin—so you’ll typically feel a quick pinch or mild sting, especially if you inject too quickly. Injecting slowly and choosing a fatty area (like the lower abdomen or outer thigh) helps minimize discomfort.

Can I mix NAD+ with other supplements?

Yes—but with caution. NAD+ is often paired with other wellness therapies, like vitamin B12 or glutathione, but combining injectables or stacking supplements should always be discussed with a provider. IVY RX can help create a safe, coordinated plan that works for your body.

How fast will I feel results from NAD+ injections?

That depends on your dose, goals, and baseline NAD+ levels. Some people notice a boost in energy or mental clarity within hours or days, especially at higher doses. Others experience more gradual improvements in sleep, recovery, and focus over a few weeks of consistent use. Your IVY RX expert can help fine-tune the dose and frequency to match your goals.

References

1. Fang EF, Lautrup S, Hou Y, Demarest TG, Croteau DL, Mattson MP, Bohr VA. NAD+ in aging: molecular mechanisms and translational implications. Trends in Molecular Medicine. 2017;23(10):899–916. https://pmc.ncbi.nlm.nih.gov/articles/PMC7494058/. doi:10.1016/j.molmed.2017.08.001
2. Zhang H, Ryu D, Wu Y, Gariani K, Wang X, Luan P, D’Amico D, Ropelle ER, Lutolf MP, Aebersold R, et al. NAD+ repletion improves mitochondrial and stem cell function and enhances life span in mice. Science. 2016;352(6292):1436–1443. https://doi.org/10.1126/science.aaf2693. doi:10.1126/science.aaf2693
3. Amjad S, Nisar S, Bhat AA, Shah AR, Frenneaux MP, Fakhro K, Haris M, Reddy R, Patay Z, Baur J, et al. Role of NAD+ in regulating cellular and metabolic signaling pathways. Molecular Metabolism. 2021;49:101195. https://pmc.ncbi.nlm.nih.gov/articles/PMC7973386/. doi:10.1016/j.molmet.2021.101195

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